Source BioScience tests all clones prior to shipment for T1 phage contamination. However, Source BioScience cannot warrant that all clones are free of T1 phage contamination. You are therefore advised to handle all clones with appropriate care.

□Handling and storage of clones

Handling and storage condition vary depending of additive antibiotics. The colour of a lid is corresponded to antibiotics. Additive antibiotic is corresponded to Clone ID.

□IRBO (Zebrafish)

Clones are cultured on LB agar medium (50 ?g/ml Ampicillin）. Store at 4°C without freezing. Pick a single colony of the clone off of a tube and grow 24 hours at 37°C in the same composition of medium as soon as possible.

□IRBV (Zebrafish)

Clones are cultured on LB agar medium （27 ?g/ml Chloramphenicol). Store at 4°C without freezing. Pick a single colony of the clone off of a tube and grow 24 hours at 30°C in the same composition of medium as soon as possible.

Culture more than ten single colonies of the each clone in the LB liquid medium with the same antibiotic and 8% glycerol, and freeze the glycerol stock at -70°C. The clone stocks are ready to culture again during the plasmid purification process.

The single colony picking is an essential steps for storage and handling of clones to avoid risks of contamination, cross contamination, unexpected mutation of original clones. With larger number of single colonies, larger possibility to recover the original clone. In the same way, you can isolate the original clone from a contaminated clone stock.