Reagents for Eprobe® PCR : Products Overview
□Eprobe® PCR
Eprobe® is a fluorescent dye attached oligonucleotide that designed to realise highly-target specific DNA/RNA detection with significant signal-to-noise ratio during Real-Time PCR process. Eprobe® allows simultaneous analysis of RT-PCR and melt curve analysis with a normal RT-PCR equipment.
DNAFORM supplies enzymes optimized for use in probe® PCR. As a original developer of the technique, we also provide custom Eprobe®/Eprimer™ synthesis service.
□E-Taq 2xPCR Mix
E-Taq 2xPCR Mix is a PCR reaction pre-mix with optimized composition for Eprobe® PCR.
□Description of E-Taq 2xPCR Mix
- □A hot start PCR enzyme：A monoclonal antibody to the polymerase binds E-Taq polymerase, thereby preventing nonspecific DNA amplification due to mispriming.
- □Stable signal level：E-Taq polymerase lacks 3'-5', 5'-3' exonuclease activity. Therefore, it does not cause degradation of Eprobe®, an oligonucleotide probe, and subsequent signal level decrease during PCR.
- □All-in-one：With reaction buffer, dNTP, Magnesium, and other required components in one tube, E-Taq 2xPCR Mix is ready-to-use. You can quickly perform Eprobe® PCR by preparing template, Eprobe®, and E-Taq 2xPCR Mix.
□Applications
- □Sensitive detection of a gene (ex. identification of causative organism) by Eprobe® PCR
- □SNP assay
- □Detection of rare genetic variant in a sample
□Specification
E-Taq 2xPCR Mix is 2x concentration. Use half volume of reaction volume, add primer, Eprobe®, and template, dilute double with distilled water.
Volume：20 μL (The quantity supports typically 100 reactions)
□E-Taq DNA Polymerase
E-Taq DNA Polymerase is a Taq DNA polymerase optimized for Eprobe®.
□Descriptions of E-Taq DNA Polymerase
- □A hot start PCR enzyme：A monoclonal antibody to the polymerase binds E-Taq polymerase, thereby preventing nonspecific DNA amplification due to mispriming.
- □Stable signal level：E-Taq polymerase lacks 3'-5', 5'-3' exonuclease activity. Therefore, it does not cause degradation of Eprobe®, an oligonucleotide probe, and subsequent signal level decrease during PCR.
□Applications
- □Sensitive detection of a gene (ex. identification of causative organism) by Eprobe® PCR
- □SNP assay
- □Detection of rare genetic variant in a sample
□Specifications
Volume：5 U/μL
With 10x buffer(dNTP is not included)
□Notes
- □This product is intended for research purposes only.
- □DNAFORM & RIKEN has joint patents for these reagents