Eprobe®/Eprimer™ is a fluorescence labeled oligonucleotide that provides target-specific signals with significant signal to noise ratio. At the single-strand state, the two fluorescence dyes attached to Eprobe®/Eprimer™ are quenched by interaction of each other. Upon hybridation to a target template, the dyes are separated and emits a strong fluorescence signal.

DNAFORM provides custom Eprobe®/Eprimer™ for your research.

□Benefits of Using Eprobe®/Eprimer™

• □High S/N ratio
• □High target specifity
• □Real-time PCR and melting curve analysis with a single probe

□Applications of Eprobe® PCR

• □Real-time PCR
• □SmartAmp® and other isothermal amplification methods
• □Melging curve analysis

□Instructions

• □Currently, fluorescent-dye-labeling at a thymidine (T) site is only available.
• □Multi-labeling is not available.
• □Thiazole orange (EX/EM: 510/530 nm) and Thiazole pink (EX/EM: 570/590 nm) are available as a dye.
• □3'-end labeling is not available
• □Avoid labeling at nucleotides positioned less than 3nt from 5'-end or 3'-end. It may cause undesired affection in reaction/detection due to change in colour, increased baseline signal, and other unpredictable effects on the labeled oligonucleotide.
  Ex.: 5'-ZAGCAGC..., 5'-AZCCGCA..., 5'-AGZCGA..., ...AGCZGC-3', ...CGAZG-3'
  (Z: dye-labeled T)
• □Maximum allowable length of a mononucleotide repeat is four (for example ...GGGG...), otherwise we cannot guarantee minimum yield 10 nmol.
• □Contact us if you need consultation about the dye position.

□Long Oligos Up to 50 Bases (consultation required)

Large scale, guaranteed yield: 10 nmol

Maximum 50 bases

□Quality Control

Purity assay with reverse phase HPLC (all products, minimum 80% purity)

Purity assay with PAGE (sampling test)