High-throughput sequencer, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping, providing researchers a wide rang of applications.

In addition to the unique libraries based on our original technologies, DNAFORM also provides flexible library preparation service.

□Genome-Seq

DNAFORM constructs a library for genome sequence according to the applications.

• □Whole genome sequence
  A library for optimized for whole-genome sequencing. Genome-wide variation analysis is one of its major applications.

  
  

• □De-novo sequence
  De novo genome sequencing is used to sequence uncharacterized genomes where there is no reference sequence available, or known genomes where significant structural variation is expected.

  
  

• □Target resequencing
  Targeted resequencing involves isolation of genomic regions of interest in a sample library, that typically investigate a few (or few dozen) genes to detect germline and somatic variants across large populations.

□ChIP-Seq

Combining chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing, ChIP-Seq allows genome-wide identification of the DNA-associated protein binding sites, It is the effective technique to investigate genomic region involved in gene regulation or chromatin organization.

□RNA-Seq

• □Directional RNA-Seq

  
  

• □Non-directional RNA-Seq
  RNA-Seq is utilized for quantitative investigation of transcripts and their structure. Non-directional provides information on sequence data of a novel transcript, as well as novel splice variant. Moreover, non-direct RNA-Seq allows detection of anti-sense RNAs that was not available by the directional RNA-Seq.

□Small RNA-Seq

Small RNA-Seq is utilized for the discovery and profiling of microRNAs and other non-coding RNA on any organism.